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dc.contributor.authorLacková, Zuzana
dc.contributor.authorBuchtelová, Hana
dc.contributor.authorBuchtová, Žaneta
dc.contributor.authorKlejdus, Bořivoj
dc.contributor.authorHeger, Zbyněk
dc.contributor.authorBrtnický, Martin
dc.contributor.authorKynický, Jindřich
dc.contributor.authorZítka, Ondřej
dc.contributor.authorAdam, Vojtěch
dc.date.accessioned2022-03-17T01:02:12Z
dc.date.available2022-03-17T01:02:12Z
dc.date.issued2017
dc.identifier.issn1420-3049 Sherpa/RoMEO, JCR
dc.identifier.urihttps://repozitar.mendelu.cz/xmlui/handle/20.500.12698/1514
dc.description.abstractThis study shows the effects of spices, and their phenolic and flavonoid compounds, on prostate cell lines (PNT1A, 22RV1 and PC3). The results of an MTT assay on extracts from eight spices revealed the strongest inhibitory effects were from black pepper and caraway seed extracts. The strongest inhibitory effect on prostatic cells was observed after the application of extracts of spices in concentration of 12.5 mg.mL- 1. An LC/MS analysis identified that the most abundant phenolic and flavonoid compounds in black pepper are 3,4-dihydroxybenzaldehyde and naringenin chalcone, while the most abundant phenolic and flavonoid compounds in caraway seeds are neochlorogenic acid and apigenin. Using an MTT assay for the phenolic and flavonoid compounds from spices, we identified the IC50 value of ~1 mmol.L- 1 PNT1A. The scratch test demonstrated that the most potent inhibitory effect on PNT1A, 22RV1 and PC3 cells is from the naringenin chalcone contained in black pepper. From the spectrum of compounds assessed, the naringenin chalcone contained in black pepper was identified as the most potent inhibitor of the growth of prostate cells.en
dc.formatnestrankovano
dc.publisherMDPI AG (Multidisciplinary Digital Publishing Institute-MDPI)
dc.relation.ispartofMolecules
dc.relation.urihttps://doi.org/10.3390/molecules22101626
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject3,4-dihydroxybenzaldehydeen
dc.subjectApigeninen
dc.subjectClonogenic assayen
dc.subjectMTT assayen
dc.subjectNaringenin chalconeen
dc.subjectNeochlorogenic aciden
dc.subjectProstate canceren
dc.subjectScratch testen
dc.subjectSpicesen
dc.titleAnticarcinogenic effect of spices due to phenolic and flavonoid compounds-in vitro evaluation on prostate cellsen
dc.typeJ_ČLÁNEK
dc.date.updated2022-03-17T01:02:12Z
dc.description.versionOA
local.identifier.doi10.3390/molecules22101626
local.identifier.scopus2-s2.0-85030785430
local.identifier.wos000414670600054
local.number10
local.volume22
local.identifier.obd43913470
local.identifier.e-issn1420-3049
dc.project.IDIGA_AF_ IP_2017/033
dc.project.IDLQ1601
dc.project.IDVliv dezinfekčních prostředků používaných v chemických toaletách na proces anaerobní stabilizace čistírenských kalů a produkci bioplynu
dc.project.IDCEITEC 2020
dc.identifier.orcidLacková, Zuzana 0000-0003-4254-5992
dc.identifier.orcidBuchtelová, Hana 0000-0002-6846-7972
dc.identifier.orcidKlejdus, Bořivoj 0000-0002-5557-5771
dc.identifier.orcidHeger, Zbyněk 0000-0002-3915-7270
dc.identifier.orcidBrtnický, Martin 0000-0001-5237-722X
dc.identifier.orcidKynický, Jindřich 0000-0001-9193-8990
dc.identifier.orcidZítka, Ondřej 0000-0001-7607-5058
dc.identifier.orcidAdam, Vojtěch 0000-0002-8527-286X
local.contributor.affiliationAF
local.contributor.affiliationLDF


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Zobrazit minimální záznam

CC BY 4.0
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