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dc.contributor.authorHanišáková, Nikola
dc.contributor.authorVítězová, Monika
dc.contributor.authorVítěz, Tomáš
dc.contributor.authorKushkevych, Ivan
dc.contributor.authorKotrlová, Eva
dc.contributor.authorNovák, David
dc.contributor.authorLochman, Jan
dc.contributor.authorZavada, Roman
dc.date.accessioned2024-11-06T01:19:57Z
dc.date.available2024-11-06T01:19:57Z
dc.date.issued2023
dc.identifier.issn1664-302X Sherpa/RoMEO, JCR
dc.identifier.urihttps://repozitar.mendelu.cz/xmlui/handle/20.500.12698/1959
dc.description.abstractIn recent years, there has been a growing interest in extending the potential of underground gas storage (UGS) facilities to hydrogen and carbon dioxide storage. However, this transition to hydrogen storage raises concerns regarding potential microbial reactions, which could convert hydrogen into methane. It is crucial to gain a comprehensive understanding of the microbial communities within any UGS facilities designated for hydrogen storage. In this study, underground water samples and water samples from surface technologies from 7 different UGS objects located in the Vienna Basin were studied using both molecular biology methods and cultivation methods. Results from 16S rRNA sequencing revealed that the proportion of archaea in the groundwater samples ranged from 20 to 58%, with methanogens being the predominant. Some water samples collected from surface technologies contained up to 87% of methanogens. Various species of methanogens were isolated from individual wells, including Methanobacterium sp., Methanocalculus sp., Methanolobus sp. or Methanosarcina sp. We also examined water samples for the presence of sulfate-reducing bacteria known to be involved in microbially induced corrosion and identified species of the genus Desulfovibrio in the samples. In the second part of our study, we contextualized our data by comparing it to available sequencing data from terrestrial subsurface environments worldwide. This allowed us to discern patterns and correlations between different types of underground samples based on environmental conditions. Our findings reveal presence of methanogens in all analyzed groups of underground samples, which suggests the possibility of unintended microbial hydrogen-to-methane conversion and the associated financial losses. Nevertheless, the prevalence of methanogens in our results also highlights the potential of the UGS environment, which can be effectively leveraged as a bioreactor for the conversion of hydrogen into methane, particularly in the context of Power-to-Methane technology.en
dc.format1293506
dc.publisherFrontiers Media SA
dc.relation.ispartofFrontiers in Microbiology
dc.relation.urihttps://doi.org/10.3389/fmicb.2023.1293506
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectmethanogensen
dc.subjectmethaneen
dc.subjecthydrogen storageen
dc.subjectmethanationen
dc.subjectArchaeaen
dc.titleMicrobiological insight into various underground gas storages in Vienna Basin focusing on methanogenic Archaeaen
dc.typeJ_ČLÁNEK
dc.date.updated2024-11-06T01:19:57Z
dc.description.versionOA
local.identifier.doi10.3389/fmicb.2023.1293506
local.identifier.scopus2-s2.0-85181732360
local.identifier.wos001136552400001
local.number13 December
local.volume14
local.identifier.obd43925843
local.identifier.e-issn1664-302X
dc.identifier.orcidVítěz, Tomáš 0000-0003-4442-4481
local.contributor.affiliationAF


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Except where otherwise noted, this item's license is described as CC BY 4.0