Zobrazit minimální záznam

dc.contributor.authorMazumdar, Aninda
dc.contributor.authorHaddad, Yazan Abdulmajeed Eyadh
dc.contributor.authorMilosavljević, Vedran
dc.contributor.authorMichálková, Hana
dc.contributor.authorGuráň, Roman
dc.contributor.authorBhowmick, Sukanya
dc.contributor.authorMoulick, Amitava
dc.date.accessioned2022-04-19T00:02:19Z
dc.date.available2022-04-19T00:02:19Z
dc.date.issued2020
dc.identifier.issn2079-4991 Sherpa/RoMEO, JCR
dc.identifier.urihttps://repozitar.mendelu.cz/xmlui/handle/20.500.12698/1549
dc.description.abstractAntibiotic-resistant bacterial infections have become global issues for public health, which increases the utter need to develop alternatives to antibiotics. Here, the HSER (Homo sapiens retinoic acid receptor) peptide was designed from retinoic acid receptor responder protein 2 of Homo sapiens, and was conjugated with synthesized CQDs (carbon quantum dots) for enhanced antibacterial activity in combination, as individually they are not highly effective. The HSER-CQDs were characterized using spectrophotometer, HPLC coupled with electrospray-ionization quadrupole time-of-flight mass spectrometer (ESI-qTOF) mass spectrometer, zeta potential, zeta size, and FTIR. Thereafter, the antibacterial activity against Vancomycin-Resistant Staphylococcus aureus (VRSA) and Escherichia coli (carbapenem resistant) was studied using growth curve analysis, further supported by microscopic images showing the presence of cell debris and dead bacterial cells. The antibacterial mechanism of HSER-CQDs was observed to be via cell wall disruption and also interaction with gDNA (genomic DNA). Finally, toxicity test against normal human epithelial cells showed no toxicity, confirmed by microscopic analysis. Thus, the HSER-CQDs conjugate, having high stability and low toxicity with prominent antibacterial activity, can be used as a potential antibacterial agent.en
dc.format325
dc.publisherMDPI AG (Multidisciplinary Digital Publishing Institute-MDPI)
dc.relation.ispartofNanomaterials
dc.relation.urihttps://doi.org/10.3390/nano10020325
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectbacterial infectionsen
dc.subjectantibiotic-resistanten
dc.subjectcarbon quantum dotsen
dc.subjectantibacterial activityen
dc.subjecttoxicityen
dc.titlePeptide-Carbon Quantum Dots conjugate, Derived from Human Retinoic Acid Receptor Responder Protein 2, against Antibiotic-Resistant Gram Positive and Gram Negative Pathogenic Bacteriaen
dc.typeJ_ČLÁNEK
dc.date.updated2022-04-19T00:02:19Z
dc.description.versionOA
local.identifier.doi10.3390/nano10020325
local.identifier.scopus2-s2.0-85079652774
local.identifier.wos000522456300144
local.number2
local.volume10
local.identifier.obd43918932
local.identifier.e-issn2079-4991
dc.project.IDLQ1601
dc.project.IDEF16_025/0007314
dc.project.IDCEITEC 2020
dc.project.IDMultioborový výzkum pro zvýšení aplikačního potenciálu nanomateriálů v zemědělské praxi
dc.identifier.orcidMazumdar, Aninda 0000-0002-4985-7115
dc.identifier.orcidHaddad, Yazan Abdulmajeed Eyadh 0000-0002-7844-4336
dc.identifier.orcidMilosavljević, Vedran 0000-0003-4122-0694
dc.identifier.orcidMichálková, Hana 0000-0002-6846-7972
dc.identifier.orcidGuráň, Roman 0000-0002-2912-714X
dc.identifier.orcidMoulick, Amitava 0000-0001-5769-6748
local.contributor.affiliationAF


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Zobrazit minimální záznam

CC BY 4.0
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