Growth of Ectomycorrhizal Fungi on Inorganic and Organic Nitrogen Sources

Abstract

In forest soils, nitrogen (N) is present in inorganic and organic forms. The organic forms include monomeric amino acids, but also polymers such as chitin. Ectomycorrhizal fungi are known to take up both inorganic and organic N forms, and to depolymerize large organic compounds; however, it is unknown if the compounds are used for growth. The aim of this investigation was to determine the growth of a range of ectomycorrhizal fungi on inorganic and organic N sources. Seven ectomycorrhizal fungi and one endophyte originating from mountain regions either in Austria, Mongolia, or Slovenia were grown in in-vitro cultures containing ammonium, nitrate, or chitin. Four ectomycorrhizal fungi were used to investigate growth on amino acids. All fungi, except Paxillus involutus, utilized nitrate as a N source. All fungi also grew on both chitin and N-acetylglucosamine, the amino sugar precursor of chitin. Paxillus involutus and Melanogaster broomeanus showed enhanced growth on chitin-containing media. Amanita muscaria, Rhizopogon roseolus, and Suillus granulatus, but not Paxillus involutus, were able to utilize the amino acids glycine and glutamate, as well as the tripeptide triglycine. The ability to utilize the different N sources was independent of the origin of the fungi.