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MENDELU Repository

Welcome to the Open Repository of research and development results of the Mendel University in Brno. The repository serves to archive Open Access publications of university authors. Publications are automatically sent from the OBD system to the repository in the DSpace system.

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Recent Submissions

Access status: Open Access ,
Nutrient recovery from African catfish sludge via aerobic mineralization and assessment of its fertilization potential in hydroponic basil cultivation
(Elsevier Science BV, 2026) Harabiš, Lukáš; Patloková, Kateřina; Mareš, Jan; Pokluda, Robert
The increasing demand for sustainable fertilizer sources highlights the potential of aquaculture by-products as alternatives to conventional mineral solutions in hydroponics. This study evaluated the fertilization potential of a liquid solution produced by aerobic digestion of fish sludge from a recirculating aquaculture system with African catfish (Clarias gariepinus) for indoor cultivation of basil (Ocimum basilicum L.) in a deep water culture system. Three nutrient solutions were compared: a conventional mineral solution (HYDRO), fish excrement mineralizate obtained by aerobic digestion (FEM), and a 1:1 (v/v) mixture of both (MIX). Cultivation with FEM alone resulted in a 17.5 % reduction in fresh shoot biomass and an overall reduction in N and P content in plant biomass compared to the hydroponic control, likely due to deficient potassium and iron levels in the FEM solution. However, plants grown in the FEM solution exhibited high potassium use efficiency and lower nitrate accumulation in plant tissues. The MIX variant achieved fresh shoot biomass comparable to the mineral control, enhanced root growth (+40.3 %), and reduced nitrate accumulation (MINUS SIGN 9.4 %) while maintaining vitamin C content and total antioxidant capacity. Fluorescence in situ hybridization confirmed the presence of Bacillus spp. and Azospirillum spp. in the FEM and MIX solutions, suggesting a potential biostimulant effect that needs further investigation. These findings indicate that FEM cannot serve as a full replacement for mineral nutrient solutions; however, appropriate supplementation with mineral fertilizers can achieve growth performance comparable to or exceeding that in mineral solutions, offering a sustainable and balanced cultivation strategy.
Access status: Embargo ,
Impact of winemaking technologies on polyphenolic composition and wine microbiome
(Frontiers Media SA, 2026) Kostelníková, Karolína; Frejlichová, Lucie; Špetík, Milan; Sochor, Jiří; Eichmeier, Aleš; Baroň, Mojmír
Introduction This study investigates how different oenological practices, including spontaneous and inoculated alcoholic fermentation (AF), variations in malolactic fermentation (MLF) and ageing, are associated with changes in microbial diversity and polyphenolic profile of Sauvignon blanc wines.Methods Microbial composition was investigated through high-throughput DNA sequencing, while polyphenolic compounds were analysed using LC-MS together with total phenolic content through Folin-Ciocalteu assay and antiradical activity by DPPH assay.Results and discussion AF was associated with a pronounced homogenization of the microbiota, particularly through the dominance of Saccharomyces and stable epiphytic bacteria. At later stages, microbial communities showed notable compositional divergence, with their development closely linked to technological interventions and the extent of phenolic extraction. The pomace-fermented treatment exhibited the highest polyphenol content along with the greatest compositional heterogeneity, whereas treatments with lower phenolic loads exhibited simpler microbial profiles and the stable dominance of Leuconostoc after malolactic fermentation. Polyphenols appear to act as modulatory factor in microbial succession, with extraction intensity showing a more distinct association with community shifts than the fermentation regime itself. Overall, the study highlights that technological practices affecting phenolic extraction appear to play a notable role in the observed microbial trends and the resulting wine characteristics.
Access status: Open Access ,
Effects of tree species richness on topsoil carbon and fungal diversity in European planted mixed forests are modulated by environmental conditions
(Elsevier Science BV, 2025) Werner, Ramona; Jensen, Joel; Fransson, Petra; Baum, Christel; Sandén, Hans; Rewald, Boris; Godbold, Douglas Lawrence; Mayer, Mathias; Guillemot, Joannès; Robin, Agnès; Brancalion, Pedro H. S.; Koricheva, Julia; Ponette, Quentin; Muys, Bart; Verheyen, Kris; Scherer-Lorenzen, Michael; Bauhus, Jürgen; Beyer, Friderike; Hajek, Peter; Jactel, Hervé; Weih, Martin
Mixed-species forests have emerged as a promising approach to mitigate climate change impacts through enhanced carbon (C) sequestration while maintaining productivity, biodiversity, and other ecosystem services. However, we still have a poor understanding of the context-dependency of soil C sequestration in tree mixtures, particularly how it is influenced by plant-soil-microbe interactions and environmental conditions. Using soil samples collected from nine European sites within the global network of tree diversity experiments, TreeDivNet, we examined how tree species richness is associated with topsoil C stocks, fungal community composition and diversity, and their interactions. We further investigated the influence of biotic, edaphic, and climatic factors on the relationship between tree richness and topsoil C stocks. We hypothesised that increased tree species richness leads to increased topsoil C stocks and fungal diversity, and that this effect is modulated by site-specific interactions between biotic and abiotic factors. Overall, we found topsoil C stocks in stands with high tree diversity to be greater than in monocultures across the study sites. Lower soil fertility, cooler mean annual temperatures, and lower interannual variability of temperature and precipitation were found to correlate with positive effects of tree diversity on soil C stocks. While tree diversity did not directly influence fungal diversity, topsoil C stocks were positively correlated to fungal species richness. In addition, fungal richness showed a positive correlation with the net diversity effect of tree mixtures on topsoil C, suggesting that fungal diversity may be one of several factors contributing to the context-dependency of tree diversity effects on soil C stocks. Our study shows that tree species diversity can increase topsoil C storage across Europe, influenced both directly and indirectly by fungal diversity and environmental conditions. The mediation of direct and indirect linkages between tree diversity, fungal diversity and topsoil C stocks by local abiotic context highlights the need to improve our mechanistic understanding for site-specific management of soil C sequestration in tree mixtures to promote climate change mitigation in European forests.
Access status: Open Access ,
Diversity, root tip vitality and soil-driven variations of Ectomycorrhizal communities in Pinus cembra forests from alpine treelines
(Springer International Publishing AG, 2026) Lan, Hangyu; Gorfer, Markus; Otgonsuren, Burenjargal; Godbold, Douglas Lawrence
Background and aims: Swiss stone pine (Pinus cembra) is dominant tree species in the treeline ecotone of the European Alps. Ectomycorrhizal (EM) fungi are often used in treeline reestablishment with Pinus cembra, yet our understanding of natural EM communities is limited. Methods: In three alpine regions of the Austrian Alps on both silicate and calcareous bedrocks we determined the EM communities on roots of Pinus cembra and related these to soil chemical properties. Results: The taxa composition of the EM communities was similar for the different sites, but the structure in terms of abundance varied greatly. The community of the calcareous sites differed from the silicate sites, and correlated to the level of exchangeable Ca. Similarly, on the silicate sites the levels of total Al and Fe influenced the community structure. Conclusion: The composition of EM fungal communities of Pinus cembra is strongly influenced by soil factors.
Access status: Open Access ,
Forest temperature buffering in pure and mixed stands: A high-resolution temporal analysis with generalized additive models
(Elsevier Science BV, 2025) Steinparzer, Matthias; Gillerot, Loïc; Rewald, Boris; Godbold, Douglas Lawrence; Haluza, Daniela; Guo, Qiwen; Vospernik, Sonja
Forests foster buffered microclimates, but causal mechanisms have rarely been studied on longer timescales and in differently diverse stands. Here, we explore temperature regulation by a young experimental forest in Austria, focusing on four common colline broadleaf species (Acer platanoides L., Tilia cordata Mill., Quercus robur L., Carpinus betulus L.) in monocultures, two- and four-species mixed stands. Air temperature was monitored in 28 forest plots for two years and compared to open-field controls. Using generalized additive models (GAMs), we investigated direct temperature offsets and lags between open-field and sub-canopy temperatures, considering diurnal and seasonal changes, and causal factors such as global mean radiation, relative air humidity, wind, and leaf area index (LAI). Forests generally had a cooling effect during the summer and a warming effect in winter, where the cooling magnitude varied with species composition and environmental conditions. Specifically, Acer platanoides and Carpinus betulus demonstrated the highest cooling capacities, and Quercus robur the lowest. Mixed species stands exhibited higher temperature buffering effects relative to monospecific stands, suggesting that species diversity in forests can increase the ability to regulate microclimates. Solar radiation, relative air hu midity, wind speed, and LAI all significantly influenced offsets. These findings are crucial for urban forestry and environmental planning, suggesting that careful selection of tree species can optimize temperature regulation, thereby improving human thermal comfort and ecosystem processes alike.