Quantification of 'Candidatus Phytoplasma prunorum' in apricot trees exhibiting uneven European stone fruit yellows symptoms

dc.contributor.authorKiss, Tomáš
dc.contributor.authorNečas, Tomáš
dc.date.accessioned2022-07-15T00:02:16Z
dc.date.available2022-07-15T00:02:16Z
dc.date.issued2022
dc.date.updated2022-07-15T00:02:16Z
dc.description.abstractThis study focused on quantification of 'Candidatus Phytoplasma prunorum' in apricot trees exhibiting uneven symptoms of European stone fruit yellows. For the research, 17 trees showing early leaf bud break in one part of the crown were selected in winter season, and 13 trees showing leafroll in older part of the shoots and no symptoms in younger part of the same shoots were selected in summer season. Absolute quantification of phytoplasmas was performed by real-time PCR in samples collected from asymptomatic and symptomatic parts. The results showed, that phytoplasma could not be always detected in asymptomatic parts, where 2 out of 17 samples from asymptomatic parts from winter season were negative while in summer season 6 out of 13 samples from asymptomatic parts were negative. Comparison of phytoplasma quantities between symptomatic and asymptomatic plant parts then showed significantly higher quantities in symptomatic parts from samples collected in summer season and no quantity difference in samples collected in winter season.en
dc.description.versionOA
dc.format31-36
dc.identifier.issn1211-8516
dc.identifier.orcidKiss, Tomáš 0000-0002-4344-4387
dc.identifier.orcidNečas, Tomáš 0000-0001-5781-2737
dc.identifier.urihttps://repozitar.mendelu.cz/xmlui/handle/20.500.12698/1603
dc.project.IDQK1920058
dc.project.IDEF16_017/0002334
dc.project.IDInovace integrované produkce teplomilného ovoce se zaměřením na zdravotní stav produkčních výsadeb a rozmnožovacího materiálu peckovin
dc.project.IDVýzkumná infrastruktura pro mladé vědce
dc.publisherMendelova univerzita v Brně
dc.relation.ispartofActa Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
dc.relation.urihttps://doi.org/10.11118/actaun.2022.003
dc.rightsCC BY-NC-ND 4.0
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectreal-time PCRen
dc.subject'Ca. P. prunorum'en
dc.subjectearly leaf bud breaken
dc.subjectleafrollen
dc.subjectESFYen
dc.subjectPrunus armeniacaen
dc.titleQuantification of 'Candidatus Phytoplasma prunorum' in apricot trees exhibiting uneven European stone fruit yellows symptomsen
dc.typeJ_ČLÁNEK
local.contributor.affiliationZF
local.identifier.doi10.11118/actaun.2022.003
local.identifier.e-issn2464-8310
local.identifier.obd43922714
local.identifier.scopus2-s2.0-85126616881
local.number1
local.volume70

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