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dc.contributor.authorRaco, Milica
dc.contributor.authorJung, Thomas
dc.contributor.authorGoncalves Horta Jung, Marília do Rosário
dc.contributor.authorChi, Nguyen Minh
dc.contributor.authorBotella Sánchez, Leticia
dc.contributor.authorSuzuki, Nobuhiro
dc.date.accessioned2024-02-01T01:03:26Z
dc.date.available2024-02-01T01:03:26Z
dc.date.issued2023
dc.identifier.issn0304-8608 Sherpa/RoMEO, JCR
dc.identifier.urihttps://repozitar.mendelu.cz/xmlui/handle/20.500.12698/1840
dc.description.abstractHere, we report the discovery and complete genome sequence of a novel virus, designated as "Phytophthora heveae alphaendornavirus 1" (PhAEV1), from a single isolate of the plant pathogenic oomycete Phytophthora heveae (kingdom Stramenipila) isolated from a tropical evergreen lowland rainforest in northern Vietnam. PhAEV1 was detected by both cellulose affinity chromatography of dsRNA and high-throughput sequencing of total RNA, and its presence and sequence were confirmed by RT-PCR and Sanger sequencing. The PhAEV1 genome, 12,820 nucleotides (nt) in length, was predicted to encode a single large polyprotein with the catalytic core domain of viral (superfamily 1) RNA helicase (HEL, amino acid [aa] positions 1,287-1,531), glycosyltransferase (GT, aa positions ca. 2,800-3,125), and RNA-directed RNA polymerase (RdRp, aa positions 3,875-4,112). PhAEV1 is the most similar to Phytophthora cactorum alphaendornavirus 3, sharing 39.4% and 39.1% nt and aa sequence identity, respectively. In addition to the first 5'-terminal AUG codon, three additional in-frame methionine codons were found in close proximity (nt 14-16, 96-98, and 176-178), suggesting potential additional translation initiation sites. Conserved RdRp motifs (A-E) similar to those detected in related endornaviruses were identified in PhAEV1, as well as in several previously described alphaendornaviruses from other Phytophthora species in which these motifs had not been identified previously. Phylogenetic analysis showed that PhAEV1 clusters with members of the genus Alphaendornavirus in the family Endornaviridae and is basal to two other alphaendornaviruses described from another oomycete, Phytophthora cactorum. PhAEV1 is the first virus reported in P. heveae.en
dc.format158
dc.publisherSpringer Wien
dc.relation.ispartofArchives of Virology
dc.relation.urihttps://doi.org/10.1007/s00705-023-05786-7
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectEndornaviridaeen
dc.subject+ssRNAen
dc.subjectmycovirusen
dc.subjectRNA-sequencingen
dc.subjectPhytophthora clade 5en
dc.subjectHTSen
dc.titleSequence and phylogenetic analysis of a novel alphaendornavirus, the first virus described from the oomycete plant pathogen Phytophthora heveaeen
dc.typeJ_ČLÁNEK
dc.date.updated2024-02-01T01:03:26Z
dc.description.versionOA-hybrid
local.identifier.doi10.1007/s00705-023-05786-7
local.identifier.scopus2-s2.0-85159186522
local.identifier.wos000986854500001
local.number6
local.volume168
local.identifier.obd43924907
local.identifier.e-issn1432-8798
dc.project.IDLDF_VP_2021047
dc.project.IDEF15_003/0000453
dc.project.IDZkoumání virů u patogenu Phytophthora heveae
dc.project.IDVýzkumné centrum pro studium patogenů z rodu Phytophthora
dc.identifier.orcidRaco, Milica 0000-0001-5663-9231
dc.identifier.orcidJung, Thomas 0000-0003-2034-0718
dc.identifier.orcidGoncalves Horta Jung, Marília do Rosário 0000-0003-2219-8647
dc.identifier.orcidBotella Sánchez, Leticia 0000-0002-6613-5405
local.contributor.affiliationLDF


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Zobrazit minimální záznam

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