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dc.contributor.authorKudělková, Martina
dc.contributor.authorPavelková, Radka
dc.contributor.authorOndrušiková, Eva
dc.contributor.authorVachůn, Miroslav
dc.date.accessioned2022-01-28T01:02:18Z
dc.date.available2022-01-28T01:02:18Z
dc.date.issued2017
dc.identifier43914184
dc.identifier.issn1211-8516 Sherpa/RoMEO, JCR
dc.identifier.urihttps://repozitar.mendelu.cz/xmlui/handle/20.500.12698/1468
dc.description.abstractThe effect of four modified mediums for apricot multiplication was observed in this study. A total number of 1864 single nodes of 20 Prunus armeniaca L.varieties were established. Explants surface was disinfected with 0.2 % mercuric chloride for 5 minutes. MS (1962) medium with 0.5 mg.l-1 BA, 0.01 mg.l-1 NAA and 0.5 mg.l-1 GA3 was used as a medium for primary culture. 'Velkopavlovická', 'Bergeron', genotype 1128 and genotype LE 2927 Š9 were successfully transferred to aseptic conditions and multiplied. Modified MS medium (1962), DKW/Juglans medium, Quoirin, Lepoivre (1977) medium and Marino et al. (1991) medium were used for multiplication. Modified MS medium and modified DKW/Juglans medium were not suitable for apricot multiplication at all and explants did not grow. The best results were observed in the case of Quoirin, Lepoivre (1977) medium with 0.4 mg. l-1 BA and 0.01 mg.l-1 NAA. Young plants multiplied well, were fresh and vital and no damage was observed. The highest number of new shoots was observed in the case of Marino et al. (1991) medium. The average growth of new shoots after the last passaging was 600 %, rate 7.33 (Velkopavlovická); 566 %, rate 7.0 (Bergeron); 475 %, rate 6.25 (1128) and 483 %, rate 6.33 (LE 2927 Š9)). However, new shoots in clusters were too dense and stunted and this medium is not recommended for apricot multiplication.en
dc.format67-72
dc.publisherMendelova univerzita v Brně
dc.relation.ispartofActa Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
dc.relation.urihttps://doi.org/10.11118/actaun201765010067
dc.rightsCC BY-NC-ND 4.0
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCulture mediumen
dc.subjectIn vitro propagationen
dc.subjectMultiplicationen
dc.subjectPlant grow regulatorsen
dc.subjectPrunus armeniacaen
dc.subjectShoot regenerationen
dc.subjectSingle node cultureen
dc.titleThe issues of apricot (Prunus Armeniaca L.) Micropropagationen
dc.typeJ_ČLÁNEK
dc.date.updated2022-01-28T01:02:18Z
dc.description.versionOA
local.identifier.doi10.11118/actaun201765010067
local.identifier.scopus2-s2.0-85015919197
local.number1
local.volume65
local.identifier.obd43914184
local.identifier.e-issn2464-8310
dc.project.IDQJ1210175
dc.project.IDVýzkum a vývoj standardních metodických postupů ozdravování ovocných dřevin a révy vinné pomocí chemoterapie in vitro kultur pro systém certifikace zdravotního stavu výsadbového materiálu.
dc.identifier.orcidOndrušiková, Eva 0000-0002-3316-4904
dc.identifier.orcidVachůn, Miroslav 0000-0001-9054-4412
local.contributor.affiliationZF


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CC BY-NC-ND 4.0
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