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dc.contributor.authorVršanská, Martina
dc.contributor.authorVoběrková, Stanislava
dc.contributor.authorJimenez Jimenez, Ana Maria
dc.contributor.authorStrmiska, Vladislav
dc.contributor.authorAdam, Vojtěch
dc.date.accessioned2021-12-14T01:02:13Z
dc.date.available2021-12-14T01:02:13Z
dc.date.issued2018
dc.identifier43914724
dc.identifier.issn1661-7827 Sherpa/RoMEO, JCR
dc.identifier.urihttps://repozitar.mendelu.cz/xmlui/handle/20.500.12698/1407
dc.description.abstractThe key to obtaining an optimum performance of an enzyme is often a question of devising a suitable enzyme and optimisation of conditions for its immobilization. In this study, laccases from the native isolates of white rot fungi Fomes fomentarius and/or Trametes versicolor, obtained from Czech forests, were used. From these, cross-linked enzyme aggregates (CLEA) were prepared and characterised when the experimental conditions were optimized. Based on the optimization steps, saturated ammonium sulphate solution (75 wt.%) was used as the precipitating agent, and different concentrations of glutaraldehyde as a cross-linking agent were investigated. CLEA aggregates formed under the optimal conditions showed higher catalytic efficiency and stabilities (thermal, pH, and storage, against denaturation) as well as high reusability compared to free laccase for both fungal strains. The best concentration of glutaraldehyde seemed to be 50 mM and higher efficiency of cross-linking was observed at a low temperature 4 degrees C. An insignificant increase in optimum pH for CLEA laccases with respect to free laccases for both fungi was observed. The results show that the optimum temperature for both free laccase and CLEA laccase was 35 degrees C for T. versicolor and 30 degrees C for F. fomentarius. The CLEAs retained 80% of their initial activity for Trametes and 74% for Fomes after 70 days of cultivation. Prepared cross-linked enzyme aggregates were also investigated for their decolourisation activity on malachite green, bromothymol blue, and methyl red dyes. Immobilised CLEA laccase from Trametes versicolor showed 95% decolourisation potential and CLEA from Fomes fomentarius demonstrated 90% decolourisation efficiency within 10 h for all dyes used. These results suggest that these CLEAs have promising potential in dye decolourisation.en
dc.formatNestrankovano
dc.publisherMDPI AG (Multidisciplinary Digital Publishing Institute-MDPI)
dc.relation.ispartofInternational Journal of Environmental Research and Public Health
dc.relation.urihttp://dx.doi.org/10.3390/ijerph15010023
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCLEAen
dc.subjectenzyme immobilizationen
dc.subjectlaccaseen
dc.subjectwhite rot fungien
dc.titlePreparation and Optimisation of Cross-Linked Enzyme Aggregates Using Native Isolate White Rot Fungi Trametes versicolor and Fomes fomentarius for the Decolourisation of Synthetic Dyesen
dc.typeJ_ČLÁNEK
dc.date.updated2021-12-14T01:02:13Z
dc.description.versionOA
local.identifier.doi10.3390/ijerph15010023
local.identifier.scopus2-s2.0-85039035138
local.identifier.wos000424121200023
local.number1
local.volume15
local.identifier.obd43914724
local.identifier.e-issn1660-4601
dc.project.IDLQ1601
dc.project.IDCEITEC 2020
dc.identifier.orcidVršanská, Martina 0000-0002-4689-1561
dc.identifier.orcidVoběrková, Stanislava 0000-0001-7099-7692
dc.identifier.orcidJimenez Jimenez, Ana Maria 0000-0003-1736-492X
dc.identifier.orcidStrmiska, Vladislav 0000-0002-7036-1640
dc.identifier.orcidAdam, Vojtěch 0000-0002-8527-286X
local.contributor.affiliationAF


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Zobrazit minimální záznam

CC BY 4.0
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