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dc.contributor.authorVyhnánek, Tomáš
dc.contributor.authorHanáček, Pavel
dc.contributor.authorŠafránková, Ivana
dc.contributor.authorDjordjević, Biljana
dc.contributor.authorBeranová, Helena
dc.contributor.authorTrojan, Václav
dc.contributor.authorHavel, Ladislav
dc.date.accessioned2021-11-17T01:02:14Z
dc.date.available2021-11-17T01:02:14Z
dc.date.issued2018
dc.identifier43915525
dc.identifier.issn1211-8516 Sherpa/RoMEO, JCR
dc.identifier.urihttps://repozitar.mendelu.cz/xmlui/handle/20.500.12698/1396
dc.description.abstractPaprika powder, chili powder, and black pepper are among the most frequently used spices in the world. The Internal Transcribed Spacer (ITS) regions were identified by high sequence similarity with the ITS regions of many microscopic fungi, especially representatives of the phylum Ascomycota, from 18 different spice samples that were examined. However, supplied quality certificates indicated that 10 of the 18 samples had no contamination present and were safe for human consumption. The various genera of fungi that were identified from the spices are considered to be a food safety concern as they are able to produce mycotoxins. Qualitative detection was supplemented by positive detection of viable fungal DNA using qPCR for the genera Aspergillus/Penicillium in two paprika powder and black pepper samples. These results concurred with the control analysis using axenic cultures. The described methods can be used for routine testing of spices to provide safe spices and other products to consumers.en
dc.format927-937
dc.publisherMendelova univerzita v Brně
dc.relation.ispartofActa Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
dc.relation.urihttps://doi.org/10.11118/actaun201866040927
dc.rightsCC BY-NC-ND 4.0
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectcontaminationen
dc.subjectDNA analysisen
dc.subjectidentificationen
dc.subjectspicesen
dc.titleMolecular detection of fungi in paprika, chili powder and black pepperen
dc.typeJ_ČLÁNEK
dc.date.updated2021-11-17T01:02:14Z
dc.description.versionOA
local.identifier.doi10.11118/actaun201866040927
local.identifier.scopus2-s2.0-85054594921
local.number4
local.volume66
local.identifier.obd43915525
local.identifier.e-issn2464-8310
dc.project.IDTG02010074
dc.project.IDMENDELU Proof-of-Concept projekt
dc.identifier.orcidVyhnánek, Tomáš 0000-0002-9628-3716
dc.identifier.orcidHanáček, Pavel 0000-0002-9807-1370
dc.identifier.orcidŠafránková, Ivana 0000-0002-0186-5167
dc.identifier.orcidDjordjević, Biljana 0000-0003-2584-7876
dc.identifier.orcidBeranová, Helena 0000-0002-4266-0379
dc.identifier.orcidHavel, Ladislav 0000-0003-3378-2785
local.contributor.affiliationAF


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CC BY-NC-ND 4.0
Except where otherwise noted, this item's license is described as CC BY-NC-ND 4.0