Show simple item record

dc.contributor.authorPavelková, Petra
dc.contributor.authorKiss, Tomáš
dc.contributor.authorNečas, Tomáš
dc.date.accessioned2021-06-13T00:02:17Z
dc.date.available2021-06-13T00:02:17Z
dc.date.issued2019
dc.identifier43916972
dc.identifier.issn1211-8516 Sherpa/RoMEO, JCR
dc.identifier.urihttps://repozitar.mendelu.cz/xmlui/handle/20.500.12698/1276
dc.description.abstractEvaluation of the presence and concentration of PPV (Plum pox virus) in selected rootstocks was carried out in 2016-2017. For the purpose of the experiment we used rootstocks derived from crossbreeding of Prunus davidiana (Carr.) Franch, such as Cadaman and Barrier, and also a P. davidiana seedling. Peach seedling rootstock GF-305 was used as a control. The rootstocks were inoculated artificially with PPV strain M (Marcus). Both the rootstock and the inoculum were tested for presence of the virus by a serological method - semiquantitative DAS-ELISA test and molecular methods - RT-PCR, real-time RT-PCR and RT-LAMP. During the growing season the plants were evaluated for symptom intensity by using a scoring scale. The results show interdependency between symptom intensity and the amount of PPV in leaves, with DAS-ELISA test giving less positive samples than RT-PCR. The RT-LAMP and real-time RT-PCR methods were capable of revealing low concentrations of the virus even in symptom-free plants. The lowest PPV concentrations of all the four rootstocks were detected by real-time RT-PCR in P. davidiana. The highest PPV concentrations were detected in Barrier rootstock. In inocula, the lowest concentration was found in the inocula on Cadaman rootstock, whereas the highest PPV concentration was detected in the inocula inoculated on Barrier rootstock.en
dc.format121-131
dc.publisherMendelova univerzita v Brně
dc.relation.ispartofActa Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
dc.relation.urihttps://doi.org/10.11118/actaun201967010121
dc.rightsCC BY-NC-ND 4.0
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectDAS-ELISAen
dc.subjectDavid's peachen
dc.subjectReal-time RT-PCRen
dc.subjectRT-LAMPen
dc.subjectRT-PCRen
dc.subjectSharkaen
dc.subjectStone fruiten
dc.subjectSymptomsen
dc.titleEvaluation of presence and concentration of ppv in rootstocks derived from prunus davidiana (carr.) Franchen
dc.typeJ_ČLÁNEK
dc.date.updated2021-06-13T00:02:16Z
dc.description.versionOA
local.identifier.doi10.11118/actaun201967010121
local.identifier.scopus2-s2.0-85062531174
local.number1
local.volume67
local.identifier.obd43916972
local.identifier.e-issn2464-8310
dc.project.IDQJ1510081
dc.project.IDIGA - ZF/2017 - DP002
dc.project.IDInovace klíčových technologických postupů školkařské výroby ovocných výpěstků.
dc.project.IDVyhodnocení přítomnosti a koncentrace PPV v pletivech rostlin při použití podnoží P.davidiana, Cadaman, Barrier
dc.identifier.orcidKiss, Tomáš 0000-0002-4344-4387
dc.identifier.orcidNečas, Tomáš 0000-0001-5781-2737
local.contributor.affiliationZF


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

CC BY-NC-ND 4.0
Except where otherwise noted, this item's license is described as CC BY-NC-ND 4.0